p53 Antibody (AF6073)

Western blot analysis of extracts from 293, using p53 Antibody. Lane 1 was treated with the blocking peptide.

AF6073 at 1/100 staining human breast carcinoma by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22ºС. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

AF6073 staining Hela cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37ºС. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37ºС. A Alexa Fluor594 conjugated goat polyclonal to rabbit IgG (H+L) diluted 1/600 was used as secondary antibody.