Phospho-AurB (Thr232) Antibody (AF3475)

Western blot analysis of Phospho-AurB (Thr232) Antibody expression in Nocodazole treated COS7 cells lysates. The Lane on the right was treated with the antigen-specific peptide.

AF3475 at 1/100 staining human Bone marrow tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22ºС. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

AF3475 staining HT-1080 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37ºС. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37ºС. A AlexaFluor594 conjugated goat polyclonal to rabbit IgG (H+L) diluted 1/600 was used as secondary antibody.