Phospho-DARPP32 (Thr34) Antibody (AF3488)

AF3488 at 1/100 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22ºС. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

AF3488 staining MKN-45 cells treated with Forskolin 30 μm, 20 min by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37ºС. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37ºС. A AlexaFluor594 conjugated goat polyclonal to rabbit IgG (H+L) diluted 1/600 was used as secondary antibody.