PHOX2B Antibody (DF9730)

Western blot analysis of extracts from various samples, using PHOX2B Antibody. Lane 1: MDA-MB-231 cells, treated with blocking peptide. Lane 2: MDA-MB-231 cells; Lane 3: Mcf7.

DF9730 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (DF9730) and mouse anti-beta tubulin Ab (T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).