PIKFYVE Antibody (DF13761)

Western blot analysis of extracts from EC304 cells (heat-shock treatment) using PIKFYVE Antibody at 1/1000 dilution. Observed bands: 250 kD.

DF13761 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (#DF13761) and mouse anti-beta tubulin Ab (#T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).