PMM2 Antibody (DF12455)

Western blot analysis of extracts from HepG2 cells, using PMM2 antibody.

DF12455 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

DF12455 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.