Pyruvate Dehydrogenase E2 Antibody (DF14046)

Western blot analysis of extracts from various samples, using Pyruvate Dehydrogenase E2 Antibody. Lane 1: Raw264.7 cells, blocked with antigen-specific peptides. Lane 2: Raw264.7 cells. Lane 3: 293 cells (uv treatment). Lane 4: Hela cells. Lane 5: Raji cells.

DF14046 at 1/100 staining rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.

DF14046 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (#DF14046) and mouse anti-beta tubulin Ab (#T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).