SGK1 Antibody (AF6789)

Western blot analysis of extracts from COS-7 cells (uv treatment) using SGK1 Antibody. The Lane on the left was treated with blocking peptide.

AF6789 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (AF6789) and mouse anti-beta tubulin Ab (T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).