ZO 1 Antibody (AF5145)

Western blot analysis of ZO-1 using various lysates Lanes 1-2: Merged signal (red and green) Green - AF5145 observed at 195 kDa. Red - loading control, T0023, observed at 55 kDa. Blots were developed with Goat Anti-Rabbit IgG (H+L) FITC-conjugated (S0008) and Goat Anti-Mouse IgG (H+L) AlexaFluor594 -conjugated (S0005) secondary antibodies

AF5145 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (AF5145 1:200) and mouse anti-beta tubulin Ab (T0023 1:200) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).